&nsbp; | |||||||||||||||||||
|
|||||||||||||||||||
Research Magazine > ARCHIVE > Summer 97 > Article
by Judy Bolyard Purdy The amorphous blobs in Scott Merkle's test tubes don't look like
much yet. But given enough time, coaxing and good, old-fashioned luck, the
tiny yellow clumps one day may grow into towering American chestnut trees.
Earlier this century, American chestnuts spread their massive limbs throughout the forests of Eastern North America. But that was before the chestnut blight fungus stowed away on a shipment of young trees from Japan and nearly wiped out chestnuts from Maine to Georgia.
"Although a few have sprouted back from the original stumps or, more rarely, from mature nuts, most don't get large enough to reproduce," said Merkle, a UGA forestry professor.
A successful chestnut cloning program would enable scientists to convert a few cells into a forest of chestnut trees valued for both their beauty and their lumber. Merkle already has successfully cloned other native hardwood trees: sweetgums for the pulp and paper industry, native magnolias for plant nurseries and experimental yellow-poplars for environmental cleanup.
He and his graduate students start by cutting immature fruit or flower parts - in this case nuts - into small pieces and treating them with various combinations of plant growth regulators. Among their sources for immature chestnuts is the American Chestnut Foundation, which, along with the UGA Daniel B. Warnell School of Forest Resources, helps fund the research.
It turns out chestnut cloning is a lot harder than Merkle had anticipated. Merkle said he thinks the problem stems from the large size of chestnut fruit compared with other trees he has cloned. Since 1990, his group has been trying to find the precise combination of laboratory conditions that will unlock the chestnut's genetic codes at just the right times.
"The big bottleneck is going from an embryo to early plantlet growth," Merkle said. "We've never gotten the embryos to look normal. It's hard to get them to root, much less develop shoots." Because chestnut blight is still a threat, long-term survival of chestnuts also could depend on finding and then inserting a blight-resistance gene into the clones. Merkle's team has successfully inserted foreign genetic material into chestnut clones in anticipation of the day when such a gene is found. They use a gene "gun" to bombard embryos with microscopic particles of gold coated with bacterial genes, including marker genes that enable scientists to know which embryos contain the inserted genes.
Another approach to long-term survival could lie in cloning chestnut trees that already are blight-resistant, such as American chestnuts crossbred with blight-resistant Chinese chestnuts. Merkle recently started a new program to grow embryo cells using flower parts from trees produced from just such crosses.
For more information access http://www.uga.edu/~wsfr/, or e-mail Scott Merkle at smerkle@uga.cc.uga.edu.
|
|
|